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Matrix degradation in human immunodeficiency virus type 1-associated tuberculosis and tuberculosis immune reconstitution inflammatory syndrome: a prospective observational study

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dc.date.accessioned 2018-03-12T15:22:21Z
dc.date.available 2018-03-12T15:22:21Z
dc.date.issued 2017-06-29 en
dc.identifier.uri http://hdl.handle.net/20.500.11910/10942
dc.description.abstract Extensive immunopathology occurs in human immunodeficiency virus (HIV)/tuberculosis (TB) coinfection, but the underlying molecular mechanisms are not well-defined. Excessive matrix metalloproteinase (MMP) activity is emerging as a key process but has not been systematically studied in HIV-associated TB. We performed a cross-sectional study of matrix turnover in HIV type 1 (HIV-1) infected and uninfected TB patients and controls, and a prospective cohort study of HIV-1 infected TB patients at risk of TB immune reconstitution inflammatory syndrome (TB-IRIS), in Cape Town, South Africa. Sputum and plasma MMP concentrations were quantified by Luminex, plasma procollagen III N-terminal propeptide (PIIINP) by enzyme-linked immunosorbent assay, and urinary lipoarabinomannan (LAM) by Alere Determine TB LAM assay. Peripheral blood mononuclear cells from healthy donors were cultured with Mycobacterium tuberculosis and extracellular matrix in a 3D model of TB granuloma formation. MMP activity differed between HIV-1 infected and uninfected TB patients and corresponded with specific TB clinical phenotypes. HIV-1 infected TB patients had reduced pulmonary MMP concentrations, associated with reduced cavitation, but increased plasma PIIINP, compared to HIV-1 uninfected TB patients. Elevated extrapulmonary extracellular matrix turnover was associated with TB-IRIS, both before and during TB-IRIS onset. The predominant collagenase was MMP-8, which was likely neutrophil derived and M. tuberculosis antigen driven. Mycobacterium tuberculosis induced matrix degradation was suppressed by the MMP inhibitor doxycycline in vitro. MMP activity in TB differs by HIV-1 status and compartment, and releases matrix degradation products. Matrix turnover in HIV-1 infected patients is increased before and during TB-IRIS, informing novel diagnostic strategies. MMP inhibition is a potential host-directed therapy strategy for prevention and treatment of TB-IRIS. en
dc.format.medium Print en
dc.subject HIV/AIDS en
dc.subject TUBERCULOSIS en
dc.title Matrix degradation in human immunodeficiency virus type 1-associated tuberculosis and tuberculosis immune reconstitution inflammatory syndrome: a prospective observational study en
dc.type Journal Article en
dc.description.version Y en
dc.ProjectNumber N/A en
dc.Volume March en
dc.BudgetYear 2017/18 en
dc.ResearchGroup HIV/AIDS, STIs and TB en
dc.SourceTitle Clinical Infectious Diseases en
dc.ArchiveNumber 9797 en
dc.PageNumber Online en
dc.outputnumber 8687 en
dc.bibliographictitle Walker, N.F., Wilkinson, K.A., Meintjes, G., Tezera, L.B., Goliath, R., Peyper, J.M., Tadokera, R., Opondo, C., Coussens, A.K., Wilkinson, R.J., Friedland, J.S. & Elkington, P.T. (2017) Matrix degradation in human immunodeficiency virus type 1-associated tuberculosis and tuberculosis immune reconstitution inflammatory syndrome: a prospective observational study. <i>Clinical Infectious Diseases</i>. March:Online. en
dc.publicationyear 2017 en
dc.contributor.author1 Walker, N.F. en
dc.contributor.author2 Wilkinson, K.A. en
dc.contributor.author3 Meintjes, G. en
dc.contributor.author4 Tezera, L.B. en
dc.contributor.author5 Goliath, R. en
dc.contributor.author6 Peyper, J.M. en
dc.contributor.author7 Tadokera, R. en
dc.contributor.author8 Opondo, C. en
dc.contributor.author9 Coussens, A.K. en
dc.contributor.author10 Wilkinson, R.J. en
dc.contributor.author11 Friedland, J.S. en
dc.contributor.author12 Elkington, P.T. en


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